Sterile Cloning Cylinders

Convenient, Improved Cloning Technique

These cloning cylinders facilitate the isolation of individual cell clones, each generated from a single cell, yielding a homogenous population of cells. To isolate a clone, a thin layer of grease is applied to the bottom edge of the cylinder and then inverted over the clone of choice. After adding a small volume of Trypsin or EDTA, the dish is incubated at 37 °C (98.6 °F) briefly until cells detach. Cells are then collected from inside the cylinder and transferred to another vessel for further growth.

• Cylinders are tapered so bottom edge is wider than the top ensuring a good sealing surface
• Polystyrene cloning cylinders are supplied in sterile packs
• Three sizes: 4.7 mm I.D. x 8 mm H, 6.4 mm I.D. x 8 mm H and 9.5 mm I.D. x 11 mm H
• Packed 10 per bag, one size